منابع مشابه
P-95: Flow Cytometry Analysis of Bovine Semen:A Qualitative Study
Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...
متن کاملdetermination of ploidy levels of some populations of agropyron cristatum (poaceae) in iran by flow cytometry
flow cytometry (fcm) was used to determine the ploidy levels of six different populationsof agropyron cristatum (l.) gaertn. (poaceae) in iran, using a diploid cultivated barley (hordeumvulgare l.) as an internal reference. according to flow cytometric data, tetraploid (2n = ± 28) andhexaploid (2n = ± 42) levels were detected among the examined populations and these results weresupported by chr...
متن کاملFlow cytometry (ploidy determination, cell cycle analysis, DNA content per nucleus)
During recent years, flow cytometry has established itself as a useful, quick novel method to determine efficiently, reproducibly and at a reduced cost per sample the relative nuclear DNA content and ploidy level of a large number of species (plants and animals), that has also been used to sort cells with different traits from a mixed cell population (e.g. as when separating heterokaryons from ...
متن کاملComparison of flow and static image cytometry in the determination of ploidy.
AIMS To compare the efficiency of flow cytometry and computed image cytometry; and to see if a reliable set of guidelines regarding interpretation of histograms could be drawn up. METHODS The two methods were applied to a series of 111 formalin fixed renal cell carcinomas. Data generated by both methods were compared. RESULTS The methods agreed in 85 cases. Hypodiploidy was detected by comp...
متن کاملA survey of apomixis and ploidy levels among Poa L. (Poaceae) using flow cytometry
Poa is a complex genus taxonomically and genetically. As a result, relatively little information exists for this economically important genus with regards to reproductive mode and variability in chromosome number. We examined apomixis frequency and ploidy levels in 83 Poa accessions representing 33 species from the National Plant Germplasm System using fl ow cytometric techniques. In reproducti...
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ژورنال
عنوان ژورنال: Current Protocols in Microbiology
سال: 2018
ISSN: 1934-8525,1934-8533
DOI: 10.1002/cpmc.58